Dual-Stage Cross-Flow Filtration: Integrated Capture and Purification of Virus-Like Particles.

ABSTRACT: Virus-like particles (VLPs) are a versatile technology for the targeted delivery of genetic material through packaging and potential surface modifications for directed delivery or immunological issues. Although VLP production is relatively simple as they can be recombinantly produced using microorganisms such as Escherichia coli, their current downstream processing often relies on individually developed purification strategies. Integrating size-selective separation techniques may allow standardized platform processing across VLP purification. This study presents an innovative dual-stage cross-flow filtration (CFF) set-up for integrated capture and purification of VLPs, enabling processing solely based on the size-selective separation techniques precipitation and filtration. The 2 μm/300 kD

SUBMITTER: Dietrich A 

PROVIDER: S-EPMC11895408 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature