A Novel Screening System to Characterize and Engineer Quorum Quenching Lactonases.

ABSTRACT: N-acyl l-homoserine lactones are signaling molecules used by numerous bacteria in quorum sensing. Some bacteria encode lactonases, which can inactivate these signals. Lactonases were reported to inhibit quorum sensing-dependent phenotypes, including virulence and biofilm. As bacterial signaling is dependent on the type of molecule used, lactonases with high substrate specificity are desirable for selectively targeting species in communities. Lactonases characterized from nature show limited diversity in substrate preference, making their engineering appealing but complicated by the lack of convenient assays for evaluating lactonase activity. We present a medium-throughput lactonase screening system compatible with lysates that couples the ring opening of N-acyl l-homocysteine thiolactones

SUBMITTER: Sompiyachoke K 

PROVIDER: S-EPMC11895409 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature