Isolated Mitochondrial Transplantation as a Novel Treatment for Corneal Chemical Burns.

ABSTRACT:

Purpose

This study aimed to investigate the therapeutic potential of isolated mitochondrial transplantation for the restoration of corneal surface injury in mice after corneal chemical burn.

Methods

Mitochondria were isolated from mesenchymal stem cells via ultracentrifugation, followed by an assessment of their purity and viability. The internalization of mitochondria by human corneal epithelial cells was tracked using a live fluorescence imaging system. Apoptosis-related markers and mitochondrial function were measured by Western blotting and flow cytometry, respectively. Mitochondrial morphology was examined using confocal laser scanning microscopy. The therapeutic effects of subconjunctival administration of isolated mitochondria in vivo were evaluated by fluorescein sod

SUBMITTER: Jiang D 

PROVIDER: S-EPMC11895849 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature