Aged hippocampal single-cell atlas screening unveils disrupted neuroglial system in postoperative cognitive impairment.

ABSTRACT: Glia-neuron interaction is a crucial feature in aged hippocampus during the occurrence of postoperative cognitive impairment. However, the regulatory effects of microglia, astrocytes, and oligodendrocytes in this glia-neuron interaction, the potential mechanisms and gene targets are still to be elucidated. Here, single-cell RNA sequencing was performed to detect the perioperative genomic expression characteristics of neuroglial system in the hippocampus of aged mice, and to investigate the potential cross-cellular mechanisms and valuable treatment options for glia-neuron interaction-related cognitive impairment. We found that postoperative neurons and glia cells exhibited protein dysmetabolism and mitochondrial electron misrouting. Impaired autophagy and circadian rhythm worsened microglia

SUBMITTER: Suo Z 

PROVIDER: S-EPMC11896209 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature