Seq-Scope: repurposing Illumina sequencing flow cells for high-resolution spatial transcriptomics.

ABSTRACT: Spatial transcriptomics technologies aim to advance gene expression studies by profiling the entire transcriptome with intact spatial information from a single histological slide. However, the application of spatial transcriptomics is limited by low resolution, limited transcript coverage, complex procedures, poor scalability and high costs of initial setup and/or individual experiments. Seq-Scope repurposes the Illumina sequencing platform for high-resolution, high-content spatial transcriptome analysis, overcoming these limitations. It offers submicrometer resolution, high capture efficiency, rapid turnaround time and precise annotation of histopathology at a much lower cost than commercial alternatives. This protocol details the implementation of Seq-Scope with an Illumina NovaSeq 6000

SUBMITTER: Kim Y 

PROVIDER: S-EPMC11896753 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature