<i>VaWRKY65</i> contributes to cold tolerance through dual regulation of soluble sugar accumulation and reactive oxygen species scavenging in <i>Vitis amurensis</i>.

ABSTRACT: Although the significance of some plant WRKYs in response to cold stress have been identified, the molecular mechanisms of most WRKYs remain unclear in grapevine. In this study, we demonstrate that cold-induced expression of VaBAM3 in Vitis amurensis executes a beneficial role in enhancing resistance by the regulating starch decomposition. VaWRKY65 was identified as an upstream transcriptional activator of VaBAM3 through yeast one-hybrid library screening and validated to directly interact with the W-box region inside the VaBAM3 promoter. Transgenic Arabidopsis thaliana plants and grapevine roots overexpression VaWRKY65 exhibited improved cold tolerance along with higher BAM activity and soluble sugar levels, whereas opposite changes were observed

SUBMITTER: Meng L 

PROVIDER: S-EPMC11896968 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature