The transcription factors AdNAC3 and AdMYB19 regulate kiwifruit ripening through brassinosteroid and ethylene signaling networks.

ABSTRACT: The pivotal role of ethylene (ETH) in fruit ripening has been extensively studied; however, the function of brassinosteroids (BRs) in regulating fruit ripening remains poorly understood. Specifically, the mechanism by which BRs interact with ETH to affect kiwifruit (Actinidia deliciosa) ripening is unclear. Our research showed that 2 genes encoding transcription factors, AdNAC3 and AdMYB19, and the fruit softening gene AdEXP3 (encoding a cell wall expansion protein, expansin 3) were upregulated by ETH and downregulated by BRs. Furthermore, AdNAC3 and AdMYB19 positively regulated the activity of the AdEXP3 promoter, and AdNAC3 positively regulated the promoter activity of AdMYB19. The physical interaction between AdNAC3 and the B-box-type zinc finger protein AdBBX32 affected fruit ripening.

SUBMITTER: Yang Y 

PROVIDER: S-EPMC11896978 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature