P53 inhibits OTUD5 transcription to promote GPX4 degradation and induce ferroptosis in gastric cancer.

ABSTRACT:

Background

Gastric cancer is one of the most prevalent malignant tumors within the digestive system, and ferroptosis playing a crucial role in its progression. Glutathione peroxidase 4 (GPX4), a key negative regulator of ferroptosis, is highly expressed in gastric cancer and contributes to tumor growth. Targeting the regulation of GPX4 has emerged as a promising approach to induce ferroptosis and develop effective therapy for gastric cancer.

Methods

To confirm that OTUD5 is a deubiquitinase of GPX4 and regulates ferroptosis, we performed Western blotting, Co-IP, immunofluorescence, quantitative real-time PCR, Ub assay and flow cytometry experiments. To explore the physiological function of OUTD5, we knocked out the Otud5 gene in the mouse gastric cancer cell line (MFC) using

SUBMITTER: Zhang J 

PROVIDER: S-EPMC11897053 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature