CPT1A Alleviates Senescence and Restores Osteogenic Differentiation of BM-MSC Through SOD2 Succinylation.

ABSTRACT: Bone marrow mesenchymal stem cells (BM-MSCs) have promising prospects in bone repair and regenerative medicine. However, BM-MSCs gradually lose their original pluripotency and differentiation potential after successive passages. This study aimed to reveal the mechanism underlying the phenomenon. Western blotting, SA-β-gal staining and Alizarin red staining were used to evaluate the senescence phenotype and osteogenic differentiation ability. Mitochondrial ROS levels were detected using flow cytometry. Protein interactions and succinylation modifications were identified by using Co-IP assays. With the increase in passage times, the proliferation and osteogenic differentiation of BM-MSCs were gradually weakened, and the expression level of CPT1A was decreased. BM-MSCs with fewer passages (P1

SUBMITTER: Wang XY 

PROVIDER: S-EPMC11897055 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature