Phosphodiesterase 10A Inhibitor Modulates Right Ventricular Outflow Tract Electrophysiological Activities and Calcium Homeostasis via the cGMP/PKG Pathway.

ABSTRACT: Phosphodiesterase inhibitors regulate intracellular Ca²⁺ of cardiomyocytes through enhancing second messenger signalling. This study aimed to investigate whether TP-10, a selective phosphodiesterase10A inhibitor, modulates Ca²⁺ cycling, attenuating arrhythmogenesis in the right ventricular outflow tract (RVOT). Right ventricular tissues from New Zealand white rabbits were harvested, and electromechanical analyses of ventricular tissues were conducted. Intracellular Ca²⁺ was monitored using Fluo-3, and ionic current was recorded using patch-clamp in isolated cardiomyocytes. Tissues from RVOT exhibited a reduction in action potential duration at both 50% and 90% repolarisation following treatment with TP-10. This treatment also inhibited burst firing induced

SUBMITTER: Lin FZ 

PROVIDER: S-EPMC11897058 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature