A novel fluorescein sodium-based screening platform for the identification of sphingoid base-producing <i>Wickerhamomyces ciferrii</i> mutants.

ABSTRACT: The efficient identification of microbial strains capable of producing rare sphingoid bases, such as sphingosine and sphinganine, is critical for advancing microbial fermentation processes and addressing increasing industrial demands. Wickerhamomyces ciferrii, a non-conventional yeast, naturally overproduces tetraacetyl phytosphingosine (TAPS); however, the production of other valuable sphingoid bases, including sphingosine, sphinganine, and triacetyl sphingosine, remains a key target. In this study, we developed a novel screening method utilizing fluorescein sodium, a selective fluorescent dye that specifically reacts with non-acetylated sphingoid bases-sphinganine, sphingosine, and phytosphingosine-while exhibiting no reactivity with TAPS. A mutant library of W. ciferrii wa

SUBMITTER: Kang JS 

PROVIDER: S-EPMC11897276 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature