MUSET: set of utilities for constructing abundance unitig matrices from sequencing data.

ABSTRACT:

Summary

MUSET is a novel set of utilities designed to efficiently construct abundance unitig matrices from sequencing data. Unitig matrices extend the concept of k-mer matrices by merging overlapping k-mers that unambiguously belong to the same sequence. MUSET addresses the limitations of current software by integrating k-mer counting and unitig extraction to generate unitig matrices containing abundance values, as opposed to only presence-absence in previous tools. These matrices preserve variations between samples while reducing disk space and the number of rows compared to k-mer matrices. We evaluated MUSET's performance using datasets derived from a 618-GB collection of ancient oral sequencing samples, producing a filtered unitig matrix that records abundances in <10 h and 20 G

SUBMITTER: Vicedomini R 

PROVIDER: S-EPMC11897428 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature