Sequencing of high-frequency mutated genes in breast cancer (BRCA) and associated-functions analysis.

ABSTRACT:

Objective

Mutations or aberrant expression of genes in an organism tend not to be completely random and this cumulative effect predisposes to the development of malignant tumours. This study aims to reveal the possible aberrant expression of high frequency mutated genes, and then to investigate their role in development, prognosis, signalling pathway function and drug resistance in breast cancer.

Methods

The mutated genes in breast cancer (BRCA) clinical samples were identified and detected by high-throughput sequencing. High-frequency mutant genes were counted. Gene expression profiles and the relationship with prognosis were analysed throughout TCGA database. qRT-PCR was used to analyse the mRNA levels of the six high-frequency mutant genes in BRCA tissues and cell lines.

SUBMITTER: Li X 

PROVIDER: S-EPMC11897711 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature