Biohybrid Nanosystem Fabricated with Marine Diatom <i>Thalassiosira pseudonana</i> for Uric Acid Detection.

ABSTRACT: Due to the intense demand for low-cost, environmentally friendly, and stable uric acid (UA) detection methods, a novel biosensing nanosystem made with marine diatom was studied. Reduced by live diatom (Thalassiosira pseudonana), metallic nanoparticles (Cu_X) were hybridized with the heteronanostructure (Diatom frustule, DF), showing peroxidase activity 2.66-fold over horseradish peroxidase (HRP). To immobilize the enzyme directionally with increasing loading amounts, silaffin peptides (R₅ and T₈) were designed for tagging the urate oxidase (UoX). The enzyme loading on DF of tagged UoX was 1.76-fold (R₅) and 1.54-fold (T₈) that of untagged UoX. The activity of immobilized UoX-R₅ was 5.29-11.76-fold more than that

SUBMITTER: Yang X 

PROVIDER: S-EPMC11897952 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature