Analysis of the Transcriptional Control of <i>Bcl11b</i> in Chicken: IRF1 and GATA1 as Negative Regulators.

ABSTRACT: B-cell lymphoma/leukemia 11B (Bcl11b) plays roles in cell proliferation and apoptosis and holds a pivotal position within the immune system. Our previous studies have demonstrated that Bcl11b can promote cell apoptosis to curb ALV-J infection. To gain insights into the molecular mechanisms underlying Bcl11b expression regulation in chickens, we constructed various truncated dual luciferase reporter vectors and analyzed the promoter region of Bcl11b. We employed promoter-binding TF profiling assay and the dual luciferase assay of site-directed mutagenesis and the expression level of interfering or overexpressing transcription factors were used to study their transcriptional regulation mechanism of chicken Bcl11b and functions in ALV-J infection. Our findings revealed co

SUBMITTER: Qiu L 

PROVIDER: S-EPMC11898421 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature