Human-derived microRNA 21 regulates indole and L-tryptophan biosynthesis transcripts in the gut commensal <i>Bacteroides thetaiotaomicron</i>.

ABSTRACT: In the gut, microRNAs (miRNAs) produced by intestinal epithelial cells are secreted into the lumen and can shape the composition and function of the gut microbiome. Crosstalk between gut microbes and the host plays a key role in irritable bowel syndrome (IBS) and inflammatory bowel diseases, yet little is known about how the miRNA-gut microbiome axis contributes to the pathogenesis of these conditions. Here, we investigate the ability of miR-21, a miRNA that we found decreased in fecal samples from IBS patients, to associate with and regulate gut microbiome function. When incubated with the human fecal microbiota, miR-21 revealed a rapid internalization or binding to microbial cells, which varied in extent across different donor samples. Fluorescence-activated cell sorting and sequencing o

SUBMITTER: Flanagan K 

PROVIDER: S-EPMC11898669 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature