<i>mpt64</i> mutations in <i>Mycobacterium tuberculosis</i> with negative MPT64 antigen assay results from a tertiary hospital in Southeastern China.

ABSTRACT:

Background

MPT64 protein is an effective marker for detecting Mycobacterium tuberculosis (MTB) in liquid culture and clinical tissue samples. However, some MTB clinical isolates test negative for this antigen because of varied mutation types across different regions.

Methods

DNA samples of MPT64 antigen assay-negative MTB strains were collected from a tertiary hospital from January 2016 to January 2024, and mpt64 gene mutations were detected by sequencing. Clinical records of patients with negative MPT64 antigen results were collected and compared with those of patients with positive results. The global distribution of mpt64 gene mutations was analyzed using MTB genome sequences from the National Center for Biotechnology Information (NCBI) database.

R

SUBMITTER: Pan X 

PROVIDER: S-EPMC11898740 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature