Functional Characterization of <i>β</i>-Glucuronidase Genes Involved in Baicalein Biosynthesis from <i>Scutellaria baicalensis</i> Based on Transcriptome Analysis.

ABSTRACT: Baicalein is a unique flavonoid compound with important pharmacological activities, derived from Scutellaria baicalensis Georgi. Baicalein, as the aglycone of baicalin, is a key form for exerting pharmacological activity in vivo. β-glucuronidases (GUSs) are the enzymes involved in the conversion of baicalin to baicalein. In this study, the content of baicalein in S. baicalensis was significantly increased by 20.44% after treatment with 5% PEG6000. Seven GUSs from the glycoside hydrolase 79 family were identified through comparative transcriptome analysis. Among them, GUS1 and GUS2 were confirmed to have catalytic activity in converting baicalin to baicalein in prokaryotic and eukaryotic systems. The correlation analysis further revealed a significant positive co

SUBMITTER: Zuo X 

PROVIDER: S-EPMC11898752 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature