Using fluorescently labeled wheat germ agglutinin to track lipopolysaccharide transport to the outer membrane in <i>Escherichia coli</i>.

ABSTRACT: The cell envelope of gram-negative bacteria consists of two membranes sandwiching the peptidoglycan (PG) cell wall. The outer membrane (OM) contains integrated beta-barrel proteins and has an outer leaflet composed of lipopolysaccharide (LPS). LPS is transported from the inner membrane where it is made to the OM surface by the Lpt system. In the polarly elongating alpha-proteobacterium Brucella abortus, LPS transport has been localized to the polar growth zone and division site. However, LPS transport has not been tracked in live proteobacteria like Escherichia coli that elongate by dispersed incorporation of envelope material along their cell body. Here, we report an investigation into the binding target of fluorescently labeled wheat germ agglutinin (FL-WGA) on E. coli</

SUBMITTER: Dubois L 

PROVIDER: S-EPMC11898776 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature