Development and Evaluation of a New Measles Detection Assay Using Real-Time RT-PCR.

ABSTRACT: The severity of MeV infection has been greatly reduced by the development of a live attenuated vaccine, which has been incorporated into vaccination programs in many countries. However, poor access to health facilities, and above all, the increase in anti-vaccination movements, has prevented the achievement of sufficient vaccination coverage. In outbreak scenarios, a rapid and transportable method can improve differential diagnosis, including removing ambiguity in suspected measles cases, contacts, or a cohort. In response to the need, we have developed a new RT-qPCR-based MeV detection assay. The LOD of the developed assay was determined on different PCR machines and the higher threshold was 1-1.2 10³ copies/mL. The joint diagnostic sensitivity of ELISA and RT-PCR (used togethe

SUBMITTER: Chayeb VA 

PROVIDER: S-EPMC11898969 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature