Single-cell analysis identifies MKI67⁺ microglia as drivers of neovascularization in proliferative diabetic retinopathy.

ABSTRACT:

Background

Proliferative diabetic retinopathy (PDR) is among the primary causes of blindness in individuals with diabetes. Elevated lactate levels have been identified as a critical biomarker associated with the prognosis of PDR. While significant lactate accumulation has been observed in the vitreous fluid of PDR patients, the detailed pathways through which lactate impacts pathological neovascularization remain insufficiently elucidated.

Methods

The study employed single-cell RNA sequencing (scRNA-seq) to identify and characterize lactate-associated cell type in PDR patients. Key gene expression profiles and molecular pathways associated with lactate metabolism were analyzed. In vitro experiments were conducted using microglial cell cultures treated with high-glucose condi

SUBMITTER: Zou K 

PROVIDER: S-EPMC11899098 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature