Caki-1 Spheroids as a Renal Model for Studying Free Fatty Acid-Induced Lipotoxicity.

ABSTRACT: Lipotoxicity, resulting from the buildup of excess lipids in non-adipose tissues, is increasingly recognized as a major contributor to the progression of kidney disease, highlighting the need for alternative models to assess its effects on renal cells. The main aim of this study was to investigate the usefulness of Caki-1, a human proximal tubule (PT) and renal cell carcinoma (RCC) representative cell line, as a 3D model system for studying free fatty acid-induced PT lipotoxicity. Caki-1 spheroids were generated and maintained on ultra-low attachment plates and characterized regarding time-dependent morphology changes. In optimal 3D culture conditions, Caki-1 cells formed well-defined large compact spheroids with uniform morphology, good circularity, and increased diameter from days 4-12.

SUBMITTER: Battle D 

PROVIDER: S-EPMC11899473 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature