Atrophic C2C12 Myotubes Activate Inflammatory Response of Macrophages In Vitro.

ABSTRACT:

Background

Skeletal muscle wasting is commonly observed in aging, immobility, and chronic diseases. In pathological conditions, the impairment of skeletal muscle and immune system often occurs simultaneously. Recent studies have highlighted the initiative role of skeletal muscle in interactions with immune cells. However, the impact of skeletal muscle wasting on macrophage inflammatory responses remains poorly understood.

Methods

To investigate the effect of atrophic myotubes on the inflammatory response of macrophages, we established two in vitro models to induce myotube atrophy: one induced by D-galactose and the other by starvation. Conditioned medium (CM) from normal and atrophic myotubes were collected and administered to bone marrow-derived macrophages (BMDMs) from mic

SUBMITTER: Wu C 

PROVIDER: S-EPMC11899532 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature