Expression profile and N6-methyadenosine modification of circular RNA analysis in MAFLD.

ABSTRACT:

Background

To analyze the expression patterns of circRNAs in metabolic associated fatty liver disease (MAFLD) and the regulation of m⁶A methylation on those circRNAs.

Methods

The expression profile of CircRNA in MAFLD and normal control liver tissues was analyzed by microarray. Predict the potential m⁶A sites of the differentially expression circRNAs (DECs) via the SRAMP website. The biological functions and molecular interactions of DECs were analyzed by GO and KEGG analyses. The selected DECs were verified by MeRIP-qPCR and RT-qPCR.

Results

There were 59 DECs in MAFLD liver tissues compared with normal control liver tissues. We found that m⁶A sites with high or very high confidence were present in 39 of these DECs. Four randomly sele

SUBMITTER: Zheng M 

PROVIDER: S-EPMC11899672 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature