Genetic Diversity Assessment and Core Germplasm Screening of Blackcurrant (<i>Ribes nigrum</i>) in China via Expressed Sequence Tag-Simple Sequence Repeat Markers.

ABSTRACT: Blackcurrant (Ribes nigrum L.) has high nutritional value for human health due to its abundant vitamin C, flavonoids, and organic acids. However, its breeding and genetic research have been severely hindered by the lack of scientific tools such as molecular markers. Here, we identified 14,258 EST-SSR loci from 9531 CDS sequences with lengths greater than 1 kb, which comprised 6211 mononucleotide repeats, 4277 dinucleotide repeats, and 2469 trinucleotide repeats. We then randomly selected 228 EST-SSR loci for PCR amplification and gel electrophoresis imaging in the Ribes collection of Northeast Agricultural University (95 blackcurrant cultivars and 12 other Ribes accessions). As a result, 31 pairs of markers produced clear and reproducible bands of the expected size. Ba

SUBMITTER: Sun X 

PROVIDER: S-EPMC11899734 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature