Polyethylene Glycolylation of the Purified Basic Protein (Protamine) of Squid (<i>Symplectoteuthis oualaniensis</i>): Structural Changes and Evaluation of Proliferative Effects on Fibroblast.

ABSTRACT: In recent years, arginine-rich basic proteins have garnered significant attention due to their essential roles in various biological processes. However, the potential of marine-derived proteins in this domain remains largely unexplored. This study presents, for the first time, the isolation and purification of a 14.3 kDa protamine (SOP) from the mature spermatogonial tissues of Symplectoteuthis oualaniensis. Additionally, we obtained an 18.5 kDa PEGylated derivative, SOP-PEG. The physicochemical properties of both SOP and SOP-PEG were comprehensively characterized using SEM, FTIR, CD, and TGA. PEGylation markedly altered the surface morphology, secondary structure, and thermal stability of SOP. In vitro studies demonstrated that PEGylation significantly enhanced the biocompatibility

SUBMITTER: Li N 

PROVIDER: S-EPMC11899872 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature