Inhibition of LncRNA <i>H19</i> Attenuates Testicular Torsion-Induced Apoptosis and Preserves Blood-Testis Barrier Integrity.

ABSTRACT: Testicular torsion is a common emergency in adolescents, and can lead to severe ischemia reperfusion injury (IRI). LncRNA H19 has been shown to increase during ischemia, but its role in testicular IRI remains unknown. Focusing on this research gap, we utilized H19 biallelic mutant mice and Sertoli cell line (TM4) to construct in vivo and in vitro models of ischemia/reperfusion (I/R) and oxygen-glucose deprivation/reperfusion (OGD/R). Compared to WT I/R mice, H19^-/- I/R mice showed milder tissue disorganization and cell loss, with a more intact blood-testis barrier (BTB). The cell viability decreased, ROS levels and apoptosis-related factors such as Bax/Bcl-2 increased in TM4 cells after OGD/R, whereas these changes were reversed when H19 was knocked d

SUBMITTER: Cheng L 

PROVIDER: S-EPMC11899958 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature