Systematic Characterization and Analysis of the Freeze-Thaw Tolerance Gene Set in the Budding Yeast, <i>Saccharomyces cerevisiae</i>.

ABSTRACT: The budding yeast Saccharomyces cerevisiae is a widely utilized model system with myriad applications in terms of industrial, biotechnology, and synthetic biology purposes. One such application is the biosynthesis of commercially and medically important bioactive compounds and their precursors, which oftentimes require culturing conditions at low temperatures to optimize production yield rather than cellular fitness. To lend insight into genetic modifications that may assist this goal, this work focuses on a systematic analysis of the genes that result in an increase in survival following freezing. At present, these genes have been identified in a wide variety of S. cerevisiae wild-type backgrounds-that vary significantly in their properties and behaviors-and in the condition

SUBMITTER: Hartnett D 

PROVIDER: S-EPMC11900043 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature