Functionally distinct SNARE motifs of SNAP25 cooperate in SNARE assembly and membrane fusion.

ABSTRACT: Intracellular membrane traffic involves controlled membrane fission and fusion and is essential for eukaryotic cell homeostasis. Most intracellular fusion is facilitated by Soluble N-ethylmaleimide sensitive factor attachment protein receptor (SNARE) proteins, which catalyze membrane merging by assembly of a coiled helical bundle of four 60- to 70-residue "SNARE motifs." Perhaps no intracellular fusion reaction is as tightly regulated as that at the neuronal synapse, mediated by the synaptic vesicle SNARE Synaptobrevin-2 and the presynaptic plasma membrane SNAREs Syntaxin-1a and SNAP25. SNAP25 is different from its partner SNAREs: it contributes not one but two SNARE motifs to the final complex and instead of transmembrane domains is anchored in the membrane by post-translational palmitoyl

SUBMITTER: Kraichely KN 

PROVIDER: S-EPMC11900178 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature