Migrasome formation is initiated preferentially in tubular junctions by membrane tension.

ABSTRACT: Migrasomes, the vesicle-like membrane microstructures, arise on the retraction fibers (RFs), the branched nanotubules pulled out of cell plasma membranes during cell migration and shaped by membrane tension. Migrasomes form in two steps: a local RF bulging is followed by a protein-dependent stabilization of the emerging spherical bulge. Here, we addressed theoretically and experimentally the previously unexplored mechanism of bulging of membrane tubular systems. We assumed that the bulging could be driven by increases in membrane tension and experimentally verified this hypothesis in live-cell and biomimetic systems. We exposed RF-generating live cells to a hypotonic medium, which produced water flows into the cells and a related increase in the membrane tension. We observed the formation

SUBMITTER: Zucker B 

PROVIDER: S-EPMC11900186 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature