<i>ZmCaM2-1</i>, a Calmodulin Gene, Negatively Regulates Drought Tolerance in Transgenic <i>Arabidopsis</i> Through the ABA-Independent Pathway.

ABSTRACT: Calmodulin (CaM) family members play crucial roles in the response to various abiotic stresses. However, the functions of CaMs in the response to drought stress in maize are unclear. In this study, a CaM gene, ZmCaM2-1, was isolated from the maize (Zea mays L.) inbred line B73. The coding sequence (CDS) of ZmCaM2-1 was 450 bp with a protein of 149 aa which contains four EF-hand motifs. The ZmCaM2-1 protein was located in the cell nucleus and membrane, and is able to bind to Ca²⁺. ZmCaM2-1 was strongly induced by drought, NaCl, and low-temperature treatments, except for abscisic acid (ABA) treatment. Overexpression of ZmCaM2-1 in Arabidopsis was found to decrease the drought tolerance with lower antioxidant enzyme activity and greater rea

SUBMITTER: Wu Z 

PROVIDER: S-EPMC11900298 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature