Structure-Function Analysis of the Self-Sufficient CYP102 Family Provides New Insights into Their Biochemistry.

ABSTRACT: Cytochromes P450 are a superfamily of heme-containing monooxygenases involved in a variety of oxidative metabolic reactions, primarily catalyzing the insertion of an oxygen atom into a C-H bond. CYP102 represents the first example of a bacterial P450 that can be classified as a type II (eukaryotic-like) P450 and functions as a catalytically self-sufficient enzyme. These unique features have made CYP102 an attractive system for studying P450 structure and function. However, an overall picture of the specific amino acid residues that are crucial to the functioning of CYP102 and the effect of mutations on the P450 structure and catalysis is yet to be reported. Such an approach will aid protein engineering approaches used to improve this enzyme. To address this research knowledge gap, we have

SUBMITTER: Padayachee T 

PROVIDER: S-EPMC11900375 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature