Insight into crRNA Processing in <i>Streptococcus mutans</i> P42S and Application of SmutCas9 in Genome Editing.

ABSTRACT: CRISPR-Cas is an adaptive immune system found in bacteria and archaea that provides resistance against invading nucleic acids. Elements of this natural system have been harnessed to develop several genome editing tools, including CRISPR-Cas9. This technology relies on the ability of the nuclease Cas9 to cut DNA at specific locations directed by a guide RNA. In addition, the nuclease activity of Cas9 requires the presence of a short nucleotide motif (5'-NGG-3' for Cas9 from Streptococcus pyogenes) called PAM, flanking the targeted region. As the reliance on this PAM is typically strict, diverse Cas9 variants recognising different PAM motifs have been studied to target a broader range of genomic sites. In this study, we assessed the potential of Cas9 from Streptococcus mutans s

SUBMITTER: Mosterd C 

PROVIDER: S-EPMC11900481 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature