Assessment of interspecies and intergeneric gene flow for the GM <i>Jatropha curcas</i> event X8#34 with high oleic acid content in seed.

ABSTRACT: GM Jatropha X8#34 was placed for transgene flow assessment in the open field trial on Semakau Island, Singapore, between 2015 and 2017 to evaluate the potential gene flow to its non-GM counterparts and related species. The trial featured the GM Jatropha event X8#34, which is characterized by high oleic acid content, marker-free, and a homozygous transgene. The study focused on cross-pollination from the GM event to non-GM plants, analyzing factors such as distance, wind and insects mediated transfer, using event-specific multiplex PCR analysis of F1 seeds. Pollen dispersal by wind was also assessed to understand the extent of distance traveled and pollen load. Our results showed the maximum observed transgene flow was 4.5%, occurring in non-GM plants located 2 meters in third quarter of 20

SUBMITTER: Sampath K 

PROVIDER: S-EPMC11901391 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature