New insights into the tonoplast architecture of plant vacuoles and vacuolar dynamics during osmotic stress.

ABSTRACT:

Background

The vegetative plant vacuole occupies >90% of the volume in mature plant cells. Vacuoles play fundamental roles in adjusting cellular homeostasis and allowing cell growth. The composition of the vacuole and the regulation of its volume depend on the coordinated activities of the transporters and channels localized in the membrane (named tonoplast) surrounding the vacuole. While the tonoplast protein complexes are well studied, the tonoplast itself is less well described. To extend our knowledge of how the vacuole folds inside the plant cell, we present three-dimensional reconstructions of vacuoles from tobacco suspension cells expressing the tonoplast aquaporin fusion gene BobTIP26-1::gfp.

Results

3-D reconstruction of the cell vacuole made possible an accurate an

SUBMITTER: Reisen D 

PROVIDER: S-EPMC1190192 | biostudies-literature | 2005 Aug

REPOSITORIES: biostudies-literature