Transmission, Spread, Longevity and Management of Hop Latent Viroid, a Widespread and Destructive Pathogen Affecting Cannabis (<i>Cannabis sativa</i> L.) Plants in North America.

ABSTRACT: Hop latent viroid (HLVd), a 256-nucleotide RNA strand with complementary base-pairing and internal stem loop structures, forms circular or rod-shaped molecules within diseased plants. RT-PCR/RT-qPCR was used to assess HLVd transmission, spread and longevity. The viroid was detected in asymptomatic stock plants and in rooted vegetative cuttings, as well as in recirculated nutrient solution sampled from propagation tables and nozzles. Plant-to-plant spread through root infection in hydroponic cultivation was demonstrated. The viroid survived for 7 days and 4 weeks, respectively, in crushed leaf extracts (sap) or dried leaves/roots at room temperature. Following stem inoculation with infectious sap, HLVd was detected in root tissues within 2-3 weeks and in the foliage within 4-6 weeks. Plants

SUBMITTER: Punja ZK 

PROVIDER: S-EPMC11902214 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature