Phytochemical Study, Cytotoxicity, and Genotoxicity of the Methanolic Extract of <i>Geranium diffusum</i> Kunth.

ABSTRACT: Geranium diffusum Kunth is a medicinal plant native to Ecuadorian highlands with limited scientific study despite its ethnopharmacological relevance. The present study investigates the phytochemical properties and cytotoxic effects of the methanolic extract of G. diffusum. Comprehensive analyses revealed a rich composition of bioactive compounds, predominantly flavonoids and rutin, isoquercetin, and isorhamnetin-3-glucoside, known for their therapeutic potential, were isolated. The extract and its solvent fractions were evaluated for cytotoxic activity against three cell lines: RKO, SW613-B3, and HCT-116. Notably, the ethyl acetate fraction exhibited cytotoxicity with an IC₅₀ value of 44.47 ± 1.02 μg/mL against the SW613-B3 cell line, indicating its significant ant

SUBMITTER: Romero-Benavides JC 

PROVIDER: S-EPMC11902229 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature