Condensin binding at distinct and specific chromosomal sites in the Saccharomyces cerevisiae genome.

ABSTRACT: Mitotic chromosome condensation is chiefly driven by the condensin complex. The specific recognition (targeting) of chromosomal sites by condensin is an important component of its in vivo activity. We previously identified the rRNA gene cluster in Saccharomyces cerevisiae as an important condensin-binding site, but both genetic and cell biology data suggested that condensin also acts elsewhere. In order to characterize the genomic distribution of condensin-binding sites and to assess the specificity of condensin targeting, we analyzed condensin-bound sites using chromatin immunoprecipitation and hybridization to whole-genome microarrays. The genomic condensin-binding map shows preferential binding sites over the length of every chromosome. This analysis and quantitative PCR validation conf

SUBMITTER: Wang BD 

PROVIDER: S-EPMC1190225 | biostudies-literature | 2005 Aug

REPOSITORIES: biostudies-literature