Research and analysis of differential gene expression in CD34 hematopoietic stem cells in myelodysplastic syndromes.

ABSTRACT:

Objective

This study aims to investigate and analyze the differentially expressed genes (DEGs) in CD34 + hematopoietic stem cells (HSCs) from patients with myelodysplastic syndromes (MDS) through bioinformatics analysis, with the ultimate goal of uncovering the potential molecular mechanisms underlying pathogenesis of MDS. The findings of this study are expected to provide novel insights into clinical treatment strategies for MDS.

Methods

Initially, we downloaded three datasets, GSE81173, GSE4619, and GSE58831, from the public Gene Expression Omnibus (GEO) database as our training sets, and selected the GSE19429 dataset as the validation set. To ensure data consistency and comparability, we standardized the training sets and removed batch effects using the ComBat algorithm,

SUBMITTER: Wang MX 

PROVIDER: S-EPMC11902259 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature