Saccharomyces cerevisiae as a model system to define the chromosomal instability phenotype.

ABSTRACT: Translocations, deletions, and chromosome fusions are frequent events seen in cancers with genome instability. Here we analyzed 358 genome rearrangements generated in Saccharomyces cerevisiae selected by the loss of the nonessential terminal segment of chromosome V. The rearrangements appeared to be generated by both nonhomologous end joining and homologous recombination and targeted all chromosomes. Fifteen percent of the rearrangements occurred independently more than once. High levels of specific classes of rearrangements were isolated from strains with specific mutations: translocations to Ty elements were increased in telomerase-defective mutants, potential dicentric translocations and dicentric isochromosomes were associated with cell cycle checkpoint defects, chromosome fusions were

SUBMITTER: Putnam CD 

PROVIDER: S-EPMC1190249 | biostudies-literature | 2005 Aug

REPOSITORIES: biostudies-literature