Surface-Imprinted Acrylamide Polymer-Based Reduced Graphene-Gold Sensor in Rapid and Sensitive Electrochemical Determination of αB-Conotoxin.

ABSTRACT: The quantitative determination of conotoxins has great potential in the development of natural marine peptide pharmaceuticals. Considering the time-consuming sample pretreatment and expensive equipment in MS or LC-MS/MS analysis, an electrochemical sensor combined with molecularly imprinted polymer (MIP) is fabricated for the rapid monitoring of conotoxin αB-VxXXIVA to promote its pharmaceutical value and eliminate the risk of human poisoning. Electrochemically reduced graphene oxide-gold composite (rGO-Au) is modified with chitosan (CS) and glutaraldehyde (GA) to immobilize the macromolecular peptide, conotoxin αB-VxXXIVA. Subsequently, acrylamide (AAM) with a cross-linking agent, N,N'-methylene-bisacrylamide (NNMBA), is introduced into the rGO-Au electrode to form MIPs by electro-polymer

SUBMITTER: Cao J 

PROVIDER: S-EPMC11902586 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature