Role for PSF in mediating transcriptional activator-dependent stimulation of pre-mRNA processing in vivo.

ABSTRACT: In a recent study, we provided evidence that strong promoter-bound transcriptional activators result in higher levels of splicing and 3'-end cleavage of nascent pre-mRNA than do weak promoter-bound activators and that this effect of strong activators requires the carboxyl-terminal domain (CTD) of RNA polymerase II (pol II). In the present study, we have investigated the mechanism of activator- and CTD-mediated stimulation of pre-mRNA processing. Affinity chromatography experiments reveal that two factors previously implicated in the coupling of transcription and pre-mRNA processing, PSF and p54(nrb)/NonO, preferentially bind a strong rather than a weak activation domain. Elevated expression in human 293 cells of PSF bypasses the requirement for a strong activator to promote efficient splic

SUBMITTER: Rosonina E 

PROVIDER: S-EPMC1190332 | biostudies-literature | 2005 Aug

REPOSITORIES: biostudies-literature