Engineering a genomically recoded organism with one stop codon.

ABSTRACT: The genetic code is conserved across all domains of life, yet exceptions have revealed variations in codon assignments and associated translation factors^1-3. Inspired by this natural malleability, synthetic approaches have demonstrated whole-genome replacement of synonymous codons to construct genomically recoded organisms (GROs)^4,5 with alternative genetic codes. However, no efforts have fully leveraged translation factor plasticity and codon degeneracy to compress translation function to a single codon and assess the possibility of a non-degenerate code. Here we describe construction and characterization of Ochre, a GRO that fully compresses a translational function into a single codon. We replaced 1,195 TGA stop codons with the synonymous TAA in ∆TAG Escherichia co

SUBMITTER: Grome MW 

PROVIDER: S-EPMC11903333 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature