Rpm2p, a component of yeast mitochondrial RNase P, acts as a transcriptional activator in the nucleus.

ABSTRACT: Rpm2p, a protein subunit of yeast mitochondrial RNase P, has another function that is essential in cells lacking the wild-type mitochondrial genome. This function does not require the mitochondrial leader sequence and appears to affect transcription of nuclear genes. Rpm2p expressed as a fusion protein with green fluorescent protein localizes to the nucleus and activates transcription from promoters containing lexA-binding sites when fused to a heterologous DNA binding domain, lexA. The transcriptional activation region of Rpm2p contains two leucine zippers that are required for transcriptional activation and are conserved in the distantly related yeast Candida glabrata. The presence of a mitochondrial leader sequence does not prevent a portion of Rpm2p from locating to the nucleus, and se

SUBMITTER: Stribinskis V 

PROVIDER: S-EPMC1190346 | biostudies-literature | 2005 Aug

REPOSITORIES: biostudies-literature