Uptake of small extracellular vesicles by recipient cells is facilitated by paracrine adhesion signaling.

ABSTRACT: Small extracellular vesicles (sEVs) play crucial roles in intercellular communication. However, the internalization of individual sEVs by recipient cells has not been directly observed. Here, we examined these mechanisms using state-of-the-art imaging techniques. Single-molecule imaging shows that tumor-derived sEVs can be classified into several subtypes. Simultaneous single-sEV particle tracking and observation of super-resolution movies of membrane invaginations in living cells reveal that all sEV subtypes are internalized via clathrin-independent endocytosis mediated by galectin-3 and lysosome-associated membrane protein-2C, while some subtypes that recruited raft markers are internalized through caveolae. Integrin β1 and talin-1 accumulate in recipient cell plasma membranes beneath al

SUBMITTER: Hirosawa KM 

PROVIDER: S-EPMC11903687 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature