Fatty acid addition strategy redirected the metabolic flux towards an ultra-high monensin productivity of <i>Streptomyces cinnamonensis</i>.

ABSTRACT: Monensin, a polyether ionophore antibiotic produced by Streptomyces cinnamonensis, exhibits notable anticoccidial and antitumor properties. In this study, a fatty acid addition (FAA) strategy significantly enhanced the monensin production capability of S. cinnamonensis, resulting in an unprecedented monensin titer of 17.72 g/L at 192 h, 7.36 times that of the control. Physiological assay showed the FAA markedly altered the cellular morphology, cell membrane fluidity, enzymatic activity and intracellular cofactors, thus indicating of an increased carbon flux. With transcriptional analysis at the product biosynthesis phase, 4 genes in the monensin biosynthesis cluster and 11 genes related to the oxidative stress response were observed to be upregulated. Meanwhile, genes

SUBMITTER: Zhang S 

PROVIDER: S-EPMC11903796 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature