A novel model of central precocious puberty disease: Paternal MKRN3 gene-modified rabbit.

ABSTRACT:

Background

Makorin ring finger protein 3 gene (MKRN3) gene mutation is the most common genetic cause of central precocious puberty (CPP) in children. Due to the lack of ideal MKRN3-modified animal model (MKRN3-modified mice enter puberty only 4-5 days earlier than normal mice), the related research is limited.

Methods

Therefore, the MKRN3-modified rabbit was developed using CRISPR (clustered regularly interspaced short palindromic repeats) gene editing technology. The genotype identification and phenotype evaluation of MKRN3-modified rabbits were carried out.

Results

The first estrus of MKRN3-modified female rabbits was observed ~27 days earlier than that of wild-type female rabbits, with a typical CPP phenotype. This study found increased gonadotropin releasing hormo

SUBMITTER: Chen B 

PROVIDER: S-EPMC11904109 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature