Optimizing ethanol-modified supercritical CO₂ extraction for enhanced bioactive compound recovery in hemp seed oil.

ABSTRACT: This work aimed to extract hemp seed oil using modified supercritical CO₂ with ethanol, while optimizing the overall process through response surface methodology. The effects of extraction temperature (30-60 °C), pressure (10-20 MPa), and time (120-300 min) on oil yield, total phenols (TPC), total tocopherols, oxidative stability index (OSI), total chlorophylls, total carotenoids, quality indices, and color were assessed. For a maximum yield of 28.83 g/100 g of fresh seeds, the oil was extracted at 50 °C and 20 MPa for 244 min. In addition, CO₂ modified with different proportions of ethanol (2.5-20%) under the optimized SFE conditions was also tested for enhancing phenolic compound extractability in hemp seed oil. The best proportion was 10% ethanol, which significant

SUBMITTER: Allay A 

PROVIDER: S-EPMC11904181 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature