Evaluating long-read assemblers to assemble several aphididae genomes.

ABSTRACT: Aphids are a speciose family of the Hemiptera compromising >5500 species. They have adapted to feed off multiple plant species and occur on every continent on Earth. Although economically devastating, very few aphid genomes have been sequenced and assembled, and those that have suffer low contiguity due to repeat-rich and AT-rich genomes. With third-generation sequencing becoming more affordable and approaching quality levels to that of second-generation sequencing, the ability to produce more contiguous aphid genome assemblies is becoming a reality. With a growing list of long-read assemblers becoming available, the choice of which assembly tool to use becomes more complicated. In this study, six recently released long-read assemblers (Canu, Flye, Hifiasm, Mecat2, Raven, and Wtdbg2) were

SUBMITTER: Burger NFV 

PROVIDER: S-EPMC11904405 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature