Extremophilic hemoglobins: The structure of Shewanella benthica truncated hemoglobin N.

ABSTRACT: Truncated hemoglobins (TrHbs) have an ancient origin and are widely distributed in microorganisms where they often serve roles other than dioxygen transport and storage. In extremophiles, these small heme proteins must have features that secure function under challenging conditions: at minimum, they must be folded, retain the heme group, allow substrates to access the heme cavity, and maintain their quaternary structure if present and essential. The genome of the obligate psychropiezophile Shewanella benthica strain KT99 harbors a gene for a TrHb belonging to a little-studied clade of globins (subgroup 2 of group N). In the present work, we characterized the structure of this protein (SbHbN) with electronic absorption spectroscopy and X-ray crystallography and inspected its structural inte

SUBMITTER: Martinez Grundman JE 

PROVIDER: S-EPMC11904497 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature